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Author Topic: TMB precipitation of HRP substrates in Westerns  (Read 501 times)
Luke
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« on: March 17, 2008, 03:46:19 PM »
Using TMB to precipitate HRP substrate in western blots

1.   Wash membrane in PBS after HRP-conjugate incubation step
2.   Equilibrate in 10mM sodium citrate, 10mM sodium EDTA pH 5.0 for 5 min
3.   Equilibrate in sod citrate/EDTA buffer with 1% dextran sulfate for 10 minutes
4.   Wash 3 times in citrate/EDTA buffer
5.   Develop with 0.05mg/mL TMB in citrate/EDTA buffer
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