Alternative Cell Lysis Buffers

[Luke]

Alternative Cell Lysis Buffers

Notes: A single cell-lysis buffer may not work for every application.  Considerations must be made such as what types of cells are used, the cellular location of the protein, the strength of the protein:protein interactions and solubility issues.  Protease and phosphatase inhibitors have been omitted from these recipes so be sure to add them back before use.

Lysis buffer 1
•   50mM Tris-HCl pH 8.8
•   100mM NaCl
•   5mM MgCl2
•   0.5% NP40 or IGEPAL
•   1mM EDTA

Lysis buffer 2
•   150mM NaCl
•   50mM TrisHCl
•   0.1mM CaCl2
•   1% Triton X-100